This technology was developed on the basis of microdroplet in vitro fertilization, partial resection of zona pellucida, and insemination under zona pellucida for inadequate male sperm count and abnormal fertilization. In 1992, a Belgian scientist accidentally injected a single sperm into the cytoplasm of an egg during a test-tube baby test. They found that such fertilized eggs can also develop into a normal fetus, thus creating a single injection of sperm in the cytoplasm (ICSI) technology, which later became an effective method for treating male infertility such as severe oligospermia, weak sperm, and even infertility. Patients with difficulty in sperm extraction can have an epiphyseal puncture. The doctor passes a thin needle through the skin and enters the epiphyseal sperm to obtain sperm. If there is no sperm in the aspirate, take the living tissue from the testis to separate the sperm, or use the sperm cells after starting.